The fiber photometry system is a widely used tool for detecting population-level neuronal fluorescence signals. Its main feature is the ability to record activity changes of specific neurons during defined behavioral paradigms through optical techniques. This allows real-time observation of neural projection activity while animals perform complex behaviors and provides critical insights into the functions of specific neural circuits under different behavioral conditions.
With the advent of novel optogenetic fluorescent neurotransmitter probes, the application scope of this system has been further expanded. It is now capable of recording real-time dynamic changes of neurotransmitters (such as dopamine, acetylcholine, and adenosine) within specific brain regions during animal behaviors. As a result, it has become an essential tool for in vivo studies of neurotransmitter dynamics.